The whole regiment deployed to the Boer War (1899-1902) in 1899, where its ‘C’ Squadron took part in the charge at Elandslaagte (1899) and helped relieve Ladysmith (1900). During the second phase of the war the 5th Lancers were mainly in the Eastern Transvaal and they took part in the sweep into the Vryheid district. A portion of the regiment was in Cape Colony in 1901 and 1902,

The names of the men who served in the regiment are listed below