In December 1899 the second battalion embarked for the war in South Africa. They fought at Spion Kop, the Relief of Ladysmith and during the later guerilla phase of the conflict. The 3rd and 4th Militia Battalions also served in South Africa along with two and a half companies of Volunteers. In all, over 3,500 officers and men of the regiment served in the Boer War.

The names of the men who served in the regiment are listed below